This case study demonstrates:
The A Mab heavy and light chain genes were cloned into a single vector under a strong CMV promoter. After transfection, 5,000 clones were screened using (for specific productivity) and ClonePix (for secretion rate). Clone A-Mab-7B12 was selected based on: A Mab A Case Study In Bioprocess Development
The initial Protein A step used MabSelect SuRe™ resin. Loading at 30 g A Mab/L resin captured >99% of product, but elution at pH 3.5 caused significant aggregation (from 1.5% to 7%). This case study demonstrates: The A Mab heavy
The strategic use of platform technologies coupled with targeted, data-driven optimization allowed this mAb to transition smoothly from an early-stage candidate to a commercially viable manufacturing process. 99% of product
Centrifugation (depth filtration as backup) → 0.2 µm filtration.